Webinar Part II: Environment and Sampling Instrument environment focuses on maintaining the instrument environment within an operable temperature range. Guidance is presented accounting for the instrument installation location (inside or in external conditions) and the corresponding tempera ... mehr
Real-Time Monitoring of Enzyme-Catalyzed Reactions
Enzymes, as biocatalysts, are a suitable, environmentally friendly alternative to heavy metal industrial catalysts. The “Green Chemistry” nature of enzymes as catalysts is based on a number of characteristics. Enzymes are biodegradable and typically produced through fermentation of renewable feedstocks or easily mass produced through recombinant technologies. Enzymes also catalyze chemical reactions under mild temperature (20°C-40˚C) and pH conditions (pH 5-8), and perform well in aqueous environments. The chemical, petroleum, agriculture, polymer, electronics and pharmaceutical industries rely on catalysis to optimize their chemical processes. It is estimated that at least 90% of chemicals produced rely to some extent on catalysis. In recent years researchers have increasingly focused their attention on green chemistry, including catalysis. Examples of enzyme-catalyzed industrial-scale organic reactions include: Hydrolysis Oxidation Reduction Addition – Elimination Halogenation and Dehalogenation Transesterification This webinar reviews case studies from pharma, academia and the military, showing how in situ mid-IR was used to monitor the enzyme-catalyzed reactions in real time to enable researchers to understand reaction mechanisms, and determine kinetic parameters and reduce costs.