Inline Process Analytical Technology (PAT) has become critical in ensuring process robustness and productivity in the biotechnology industry. By implementing in situ probe based particle measurement technology, real time changes can be tracked to the floc size distribution while measuring inline at full process concentrations, without the need to sample. These particle size distribution tools allow scientists to identify the optimal polymer additive type and dosage for the incoming feed. PAT enables these parameters to be quickly optimized in the laboratory and transferred to the plant. This webinar reviews the application of in situ PAT to understand the flocculation mechanism in bioprocesses, and how the real-time measurement of particle size distribution by PAT can be used to improve product purity and process efficiency. Examples include laboratory and manufacturing applications to optimize and control flocculation in bioprocesses as a means of increasing clarification throughput.